Coding

Part:BBa_K4258012:Design

Designed by: Jake Schumacher   Group: iGEM22_NDSU   (2022-09-26)


His-meffRed-Linker-CBDclos


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 795
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 795
    Illegal NotI site found at 565
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 795
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 795
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 795
    Illegal NgoMIV site found at 309
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 16
    Illegal BsaI.rc site found at 1082


Design Notes

Bpi1 and Bsa1 sites added to the ends for Golden Gate Cloning.


Source

MeffRed from BBa_K1033920 Cellulose Binding Domain from BBa_K863111

References